Platinum II Taq Hot-Start DNA Polymerase is designed for fast co-cycling of the PCR assays by two innovative technologies. First, Taq enzyme is enginereered by in vitro evolution for faster DNA ...

For decades, optimizing annealing temperatures in PCR has pushed the limits of PCR forward to more difficult tasks. At the same time, mastermixes and kits became more powerful and less prone for ...

PCR involves three core components: polymerase, primer, and template. Primers anneal to templates and create initiation sites for the polymerase. The polymerase then recruits free deoxynucleoside ...

PCR products resulting from the gradient runs for determination of (A) annealing and (B) denaturation temperatures, respectively. The appropriate gradient temperatures [°C] of both runs are inserted ...

SuperScript IV UniPrime One-Step RT-PCR System is designed to perform both cDNA synthesis and subsequent PCR amplification in a single tube. This master mix formulation combines the high processivity ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...