The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

GeneFisher—fishing for genes, perhaps? Fishing genes out of genomes? Just fishing? The subtitle, Interactive PCR Primer Design, “hooked me” (get it?) but it shouldn’t have in retrospect. After all, ...

PCR and CRF are pleased to announce the creation of virtual Partners in Learning sessions that will be broadcast during the PCR e-Course (June 25-27, 2020) and CRF’s TVT Connect meeting (June 18-28, ...

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR ...

Join Asuragen at their “Advances in Practical, Broad Coverage CFTR Testing with the AmplideX ® PCR/CE CFTR Kit” virtual event. Asuragen will dive into their new kit, which is designed to provide the ...