Definition: A restriction enzyme is a DNA-cutting protein, found mainly in bacteria and archaea, that recognizes a specific short DNA sequence and cleaves the double helix at or near that site. By ...
Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...
Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...
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