High-throughput parallel transfection techniques are a prerequisite to perform large-scale experiments, such as genome-wide RNAi screens, in mammalian tissue culture cells 1,2,3,4. In such studies, ...

Transfection protocols and the reagents used to deliver nucleic acids into cells can affect cell physiology, leading to misinterpretation of results. Using microarray analysis and cell viability ...

New ready-to-use kit combines Pluristyx’s high-quality pluripotent cells and mRNA technology with Solesis’ proprietary transfection technology to achieve nearly 100% transfection efficiency and exogen ...

In this interview, News Med talks to Hinnah Campwala about the pivotal role of transfection in modern research and drug discovery. Transfection is a tool that sees widespread use across a diverse ...

Hinnah Campwala (left) is a Principal Scientist in the Cell Biology Development Group at Sartorius BioAnalytical Instruments (Michigan, USA). With a background in drug discovery and immune cell ...

The ability to alter the genetic composition of living cells has revolutionized biology. Scientific advances, from treating genetic disorders through gene therapy to reprograming skin cells into ...

Transfection is a crucial non-viral technique for introducing DNA or RNA into cells, yet achieving high efficiency and consistency can be challenging, particularly with difficult cell types.

A Few Key Considerations Are Essential to Obtain the Best Return from Cell-Based Experiments Because optimal DNA delivery into mammalian cells depends on multiple factors, we recommend optimizing ...

Transfection remains a fundamental tool for investigating gene and protein function across diverse systems. Yet variability in constructs, cell types, and delivery methods often complicates ...

Academic research trends continue to drive transfection technology advancements. Researchers hope to use more biologically relevant yet hard-to-transfect cell models in addition to the standard ...

Gene therapy involves the transfer of genetically modified materials into a patient's cells to correct abnormal genes associated with diseases (Sun, 2017). Recent advancements in gene therapy have ...

Polyethylenimine (PEI)-assisted transfection is a mainstay in upstream processing (USP) for viral vector manufacturing. But recent developments are shifting it from a ...