His-tag protein purification with IMAC (Immobilized Metal Affinity Chromatography)
20:35
YouTubethe bumbling biochemist
His-tag protein purification with IMAC (Immobilized Metal Affinity Chromatography)
If you stick a bunch of histidines in a row (about 6 or 8 or so) and over a nickel column flow, that “His tag” will stick so your tagged protein you can “pick,” then compete it off with imidazole - it’s quite a cool trick! IMAC isn’t some new product release from Apple. But it is, at least for biochemists, even more exciting! IMAC ...
13.6K viewsJul 7, 2021
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